Expressing:Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells.
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [18], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [19], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [20], and the AR expression vector and ARR2-Luc vector were generated as previously described [21,22]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [22], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [ ], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [ ], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [ ], and the AR expression vector and ARR2-Luc vector were generated as previously described [ , ]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [ ], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
Plasmid Preparation:Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells.
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [18], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [19], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [20], and the AR expression vector and ARR2-Luc vector were generated as previously described [21,22]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [22], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [ ], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [ ], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [ ], and the AR expression vector and ARR2-Luc vector were generated as previously described [ , ]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [ ], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
Binding Assay:Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells.
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [18], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [19], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [20], and the AR expression vector and ARR2-Luc vector were generated as previously described [21,22]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [22], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [ ], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [ ], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [ ], and the AR expression vector and ARR2-Luc vector were generated as previously described [ , ]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [ ], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
Mutagenesis:Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells.
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [18], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [19], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [20], and the AR expression vector and ARR2-Luc vector were generated as previously described [21,22]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [22], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [ ], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [ ], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [ ], and the AR expression vector and ARR2-Luc vector were generated as previously described [ , ]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [ ], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
Luciferase:Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells.
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [18], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [19], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [20], and the AR expression vector and ARR2-Luc vector were generated as previously described [21,22]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [22], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [ ], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [ ], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [ ], and the AR expression vector and ARR2-Luc vector were generated as previously described [ , ]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [ ], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
Generated:Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells.
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [18], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [19], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [20], and the AR expression vector and ARR2-Luc vector were generated as previously described [21,22]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [22], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
Article Title: The Role of Crosstalk between AR3 and E2F1 in Drug Resistance in Prostate Cancer Cells
Article Snippet: Auranofin (BML-EI206-0100) was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and dissolved in DMSO (stock concentration: 200 mg/mL). .. The plasmids used in the present study are as follows: the E2F1 expression vector pCMVHA E2F1 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24225) [ ], the E2F1 DNA binding mutant vector pCMV E2F1 E132 was a gift from Kristian Helin (Addgene, Cambridge, MA, USA, plasmid # 24224) [ ], the E2F1 promoter luciferase vector pGL2-AN was a gift from William Kaelin (Addgene, Cambridge, MA, USA, plasmid # 20950) [ ], and the AR expression vector and ARR2-Luc vector were generated as previously described [ , ]. .. The antibodies used in this study include: Rabbit polyclonal anti-AR3 [ ], Anti-AR3(Clone RM7; ReMab, San Francisco, CA, USA), Anti-AR-FL (sc-815; Santa Cruz, Dallas, TX, USA), Anti-AR (sc-816; Santa Cruz, Dallas, TX, USA), Anti-α-tubulin (clone DM1A; Sigma, St Louis, MO, USA), Anti-E2F1 (clones KH20 and KH95; Millipore, Burlington, MA, USA), Anti-E2F1 (A300-766A; Sigma, St Louis, MO, USA), Anti-Rb (9309S; CST, Danvers, MA, USA), Anti-PARP1(sc-8007; Santa Cruz, Dallas, TX, USA), Anti-Caspase3 (9661S; CST, Danvers, MA, USA), Anti-GAPDH (sc-365062; Santa Cruz, Dallas, TX, USA), and Anti-Ki67 (9449; CST, Danvers, MA, USA).
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